Adenovirus-mediated gene delivery to the corneal endothelium.

Larkin D., Oral H. B., Ring C., Lemoine N., George A.

Transplantation, vol.61, pp.363-70, 1996 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 61
  • Publication Date: 1996
  • Doi Number: 10.1097/00007890-199602150-00005
  • Journal Name: Transplantation
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.363-70
  • Bursa Uludag University Affiliated: Yes

Abstract

Genetic manipulation of donor cornea prior to transplantation has the potential to modulate the allogeneic response, as well as the endothelial cell function, This study examined the feasibility of gene transfer to corneal endothelial cells using replication-defective recombinant adenoviral vectors. Adult rabbit corneas were infected with recombinant adenovirus RAd35, containing the Escherichia coli beta-galactosidase (lacZ) gene, Localization of gene transfer was assessed by histochemical staining for beta-galactosidase and recombinant protein production was quantified by a soluble assay,