In vitro Pharmacodynamics of Enrofloxacin Against an Escherichia coli gyrA Mutant


CENGİZ M., Arslan E., Sorucu A.

JOURNAL OF ANIMAL AND VETERINARY ADVANCES, vol.10, no.18, pp.2384-2388, 2011 (Peer-Reviewed Journal) identifier

  • Publication Type: Article / Article
  • Volume: 10 Issue: 18
  • Publication Date: 2011
  • Journal Name: JOURNAL OF ANIMAL AND VETERINARY ADVANCES
  • Journal Indexes: Science Citation Index Expanded
  • Page Numbers: pp.2384-2388

Abstract

The aim of this study was to investigate in vitro the pharmacodynamics of Enrofloxacin (ENR) against an Escherichia coli gyrA mutant (E. coli MT/128). Broth microdilution testing was used to determine the Minimum Inhibitory Concentration (MIC) and multi-step resistance selection was performed until reaching 1 mu g ML(-1) MIC. For time-kill experiments, colony counts were determined by plating each diluted sample onto Plate Count Agar and an integrated pharmacokinetic/pharmacodynamics area measure (log ratio area) was applied to all cfu data. A single C-T mutation was found in gyrA at codon 83. Concentration-dependent bacterial killing was observed for E. coli MT/128. Bactericidal activity for this strain was achieved within 4 h at concentrations >= 8 times the MIC with no significant regrowth by 24 h.