Hemocompatibility, cytotoxicity, and genotoxicity of poly(methylmethacrylate)/nanohydroxyapatite nanocomposites synthesized by melt blending method

YILMAZ B., DOĞAN S., ÇELİKLER KASIMOĞULLARI S.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, vol.67, no.6, pp.351-360, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 6
  • Publication Date: 2018
  • Doi Number: 10.1080/00914037.2017.1331349
  • Journal Name: INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.351-360
  • Keywords: Cytotoxicity, genotoxicity, hemocompatibility, nanocomposite, nanohydroxyapatite, PMMA, ELECTROPHORESIS COMET ASSAY, DNA-DAMAGE, IN-VITRO, HUMAN-LYMPHOCYTES, INDIVIDUAL CELLS, RESIN MONOMERS, COMPOSITE, CEMENT, TISSUE, BONE
  • Bursa Uludag University Affiliated: Yes

Abstract

The aim of this study was to show the hemocompatibility, cytotoxicity, and genotoxicity of nanocomposites that were synthesized with different molecular weights of poly(methyl methacrylate) (PMMA) and different concentrations of nanohydroxyapatite (nHAp). Different techniques to characterize the nanocomposites were used. The cytotoxicity and genotoxic effects of the polymers and nanocomposites on human lymphocytes were determined by acid phosphatase assay, viability test, and comet assay. Moreover, hemocompatibility test was performed. It was found that all of the PMMA/nHAp nanocomposites are highly hemocompatible and biocompatible, none of the nanocomposites showed a cytotoxic effect, and nHAp addition decreased the genotoxicity.