Hemocompatibility, cytotoxicity, and genotoxicity of poly(methylmethacrylate)/nanohydroxyapatite nanocomposites synthesized by melt blending method


YILMAZ B., DOĞAN S., ÇELİKLER KASIMOĞULLARI S.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, vol.67, no.6, pp.351-360, 2018 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 6
  • Publication Date: 2018
  • Doi Number: 10.1080/00914037.2017.1331349
  • Title of Journal : INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
  • Page Numbers: pp.351-360
  • Keywords: Cytotoxicity, genotoxicity, hemocompatibility, nanocomposite, nanohydroxyapatite, PMMA, ELECTROPHORESIS COMET ASSAY, DNA-DAMAGE, IN-VITRO, HUMAN-LYMPHOCYTES, INDIVIDUAL CELLS, RESIN MONOMERS, COMPOSITE, CEMENT, TISSUE, BONE

Abstract

The aim of this study was to show the hemocompatibility, cytotoxicity, and genotoxicity of nanocomposites that were synthesized with different molecular weights of poly(methyl methacrylate) (PMMA) and different concentrations of nanohydroxyapatite (nHAp). Different techniques to characterize the nanocomposites were used. The cytotoxicity and genotoxic effects of the polymers and nanocomposites on human lymphocytes were determined by acid phosphatase assay, viability test, and comet assay. Moreover, hemocompatibility test was performed. It was found that all of the PMMA/nHAp nanocomposites are highly hemocompatible and biocompatible, none of the nanocomposites showed a cytotoxic effect, and nHAp addition decreased the genotoxicity.