International Eurasian Conference on Biotechnology and Biochemistry (BioTechBioChem 2020), Ankara, Turkey, 16 - 18 December 2020, vol.1, no.218, pp.68
Myogenic determination factor 1 (MyoD1) gene is a member of the myogenic differentiation gene family which plays a crucial role in growth and muscle development. There is a lack of information about the genetic variants of this gene in Turkish Grey Steppe (Boz) cattle which is an important native cattle breed in Turkey. The present study aimed to determine the genotypic/allelic frequencies and population genetic indices of MyoD1 g.782G>A polymorphism in Turkish Grey Steppe cattle. A total of 142 purebred bulls were used. Genomic DNA was isolated from whole blood using the standard phenol-chloroform extraction method. The polymerase chain reaction-restriction fragment length polymorphism technique was used for genotyping of the g.782G>A polymorphism in exon 1 of the MyoD1 gene. Estimation of genotypic/allelic frequencies and the Hardy–Weinberg equilibrium (HWE) testing (α=0.05) were performed by using Cervus v3.0 software. Moreover, the population indexes including heterozygosity (He), the number of effective alleles (Ne), and the polymorphism information content (PIC) were calculated on the basis of allelic distribution. The fixation index (FIS) was estimated from the values of theoretical (Hthe) and experimental (Hexp) heterozygosities as follows: FIS=(Hthe-Hexp)/Hthe. Results revealed that BB was the preponderant genotype (%41.55) but the AA genotype exhibited a remarkable close frequency (% 39.44). Accordingly, allelic frequencies were very close to each other (A:0.49; B:0.51). The χ2 test showed that the corresponding MyoD1 locus did not conform to Hardy-Weinberg equilibrium (χ2=54.52; P<0.001). He, Ne, and PIC values were 0.4998, 1.9992, and 0.3749, respectively. Moreover, FIS value was found to be 0.6195. These results indicated that MyoD1 g.782G>A polymorphism is a mildly informative genetic marker for Turkish Grey Steppe cattle. MyoD1 gene has been previously identified as an important constituent of the genetic control of cattle growth. The present genotypic evaluation may be a precursor study of MyoD1 g.782G>A polymorphism in Turkish native cattle breeds.