Development of in vitro produced bovine embryos in four different culture media


Dogan İ. , Sagirkaya H., Soylu M., Nur Z. , Yerlikaya H.

INDIAN JOURNAL OF ANIMAL SCIENCES, vol.72, no.3, pp.227-229, 2002 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 72 Issue: 3
  • Publication Date: 2002
  • Title of Journal : INDIAN JOURNAL OF ANIMAL SCIENCES
  • Page Numbers: pp.227-229

Abstract

The objective of this study was to compare the developmental ability of embryos in 4 different media, CR1aa, modified CR2, BCEM-g and TCM-199. Bovine oocytes were matured and then fertilized ill vitro. A total of 915 oocytes were used for fertilization, 691 (74%) of which cleaved, The 2-cell embryos were divided into 4 groups, and cultured in 1 of the 4 different culture media. Percentage of development to morula-blastocyst stage from the 2 cell stage were determined as 46. 31, 30 and 28% for CR1aa, CR2, BCEM-g and TCM-199 culture media respectively. The development rate obtained with CR1aa was significantly higher (P<0.01) compared to that with the other media.