Immobilization of Candida antarctica Lipase A on Chitosan Beads for the Production of Fatty Acid Methyl Ester from Waste Frying Oil


AYBASTIER Ö., Demir C.

ENERGY SOURCES PART A-RECOVERY UTILIZATION AND ENVIRONMENTAL EFFECTS, vol.36, no.21, pp.2313-2319, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 21
  • Publication Date: 2014
  • Doi Number: 10.1080/15567036.2011.567233
  • Journal Name: ENERGY SOURCES PART A-RECOVERY UTILIZATION AND ENVIRONMENTAL EFFECTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2313-2319
  • Keywords: Candida antarctica, chitosan, fatty acid methyl ester, immobilization, waste frying oil, COVALENT IMMOBILIZATION, BIODIESEL PRODUCTION, RUGOSA LIPASE, HORSERADISH-PEROXIDASE, ENZYMATIC CONVERSION, HYDROGEN-PEROXIDE, FUEL, TRANSESTERIFICATION, SELECTIVITY
  • Bursa Uludag University Affiliated: Yes

Abstract

Candida antarctica lipase A was immobilized by covalent binding onto chitosan beads, and these beads were used for production of fatty acid methyl ester from waste frying canola oil. The optimum immobilization conditions were determined as enzyme concentration of 12% (v/v) and immobilization time of 24 h. Transesterification reaction was performed using enzymatic catalysis for the production of fatty acid methyl ester from waste frying oil. The results indicate that 60% yield of fatty acid methyl ester was obtained under optimum immobilization conditions. Operational stability was determined with immobilized lipase and it indicated that 74% residual activity occurred after using repeatedly for five consecutive batches of 24 h each.