Enzymatic Generation of Highly Anticoagulant Bovine Intestinal Heparin

Fu L., Li K., Mori D., Hirakane M., Lin L., Grover N., ...Daha Fazla

JOURNAL OF MEDICINAL CHEMISTRY, cilt.60, sa.20, ss.8673-8679, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 60 Sayı: 20
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1021/acs.jmedchem.7b01269
  • Dergi Adı: JOURNAL OF MEDICINAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.8673-8679
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Unlike USP porcine heparin, bovine intestinal heparin (BIH) has a low anticoagulant activity. Treatment with 6-OST-1,-3, and/or 3-OST-1 afforded two remodeled heparins that met USP heparin activity and Mw specifications. We explored the pharmacodynamics and pharmacokinetics in a rabbit model. We conclude that a modest increase in the content of 3-O-sulfo groups in BIH increases the number of antithrombin III binding sites, making remodeled BIH behave similarly to pharmaceutical heparin.