Akademik Veri Yönetim Sistemi
World Immune Regulation Meeting XIX 2025, Chur, İsviçre, 12 - 15 Mart 2025, cilt.19, ss.57, (Özet Bildiri)
Bone health depends on the balance between resorption and formation. Large defects, lacking sufficient autograft volume, require additional osteogenic materials. This study aimed to develop novel bone substitutes and examine their impact on immune cells. The PBMC cultures were designed to investigate the development of an immune response to direct contact between immunologically active cells and the tested materials. PBMC cultures were finely-tuned utilizing commercially accessible compounds, including beta-tricalcium phosphate, tyramine-modified hyaluronic acid (THA) gel, filtrated/unfiltrated bone particles (monolayer/transwell cultures), agarose, fibrin sealant (Evicel®), and their combinations. Experiments involved the incorporation of tritiated thymidine, assessing cell viability using propidium iodide in flow cytometry, and targeted proteomics by proximity extension assay. We also investigated the effects of specially synthesized peptides containing different bioactive epitopes using the same experimental workflow. A synthetic peptide (T) contained a transforming growth factor-beta (TGF-β) binding motif. As a control, a scrambled peptide (E), which has no specific biological target, was used. For comparison, the effects of Peptide E and Peptide T were evaluated alone and in combination with bone particles or Evicel®. The data demonstrated a marginal elevation in the proliferative activity of cells exposed to specific test materials, including gels like THA, agarose, or Evicel® as well as peptide E. Targeted proteomics analysis identified distinct patterns for both Evicel®-related conditions and specific peptides, highlighting matrisome-, plasmacytoma-, apoptosis- and immune response-related proteins. Next, we selected proteins related to bone remodeling from the targeted proteomics (OLINK T-92, inflammation and immune response panels) data and investigated the profiles exhibited by various conditions concerning these markers. We focused on proteins including CSF−1, IFN−gamma, IL−1 alpha, LAP TGF−beta−1, OPG, TNF, and TRANCE. PBMC response to Evicel® and also its combination with filtered bone particles point out reduced bone resorption and potentially excessive bone formation. A reduced general immune response was also observed for this combination. Furthermore, peptide E promoted higher proliferation, while peptide T induced less cell proliferation and increased cell death, likely due to its TGF-β motif, which plays a complex role in immune regulation. These findings have implications for peptide-based tissue engineering and immune modulation. In addition, gels enriched with specifically synthesized targeted peptides should also be considered another valuable option.