Akademik Veri Yönetim Sistemi
16th National – 2st International Congress of Histology and Embryology (NICHE 2024), Sakarya, Türkiye, 26 - 28 Ağustos 2024, ss.1
Modulatory
effects of rosa canina seed oil on junctional protein expression in the
duodenal mucosa of epileptic rats
Introduction:
The
intestinal barrier plays a critical role in maintaining homeostasis and immune
function, with tight junctions (TJs), adherens junctions (AJs), and desmosomes
being essential for these processes. Disruption of these junctions,
particularly under conditions of oxidative stress, can lead to increased
intestinal permeability, commonly referred to as "leaky gut," which
is associated with various pathological conditions, including epilepsy. This
study explores the impact of epilepsy on intercellular junctions within the
duodenal mucosa and evaluates the potential protective effects of Rosa Canina
Seed Oil (RSO), known for its antioxidant properties.
Aim:
The
aim of this study was to investigate the effects of epilepsy on the expression
of key intercellular junctional proteins (ZO-1, E-cadherin, and vimentin) in
the duodenal mucosa of rat models, and to assess the impact of RSO treatment on
these junctions in the context of epilepsy.
Materials
and Methods: Eighty male Sprague-Dawley rats were
divided into eight groups, including control and epilepsy groups, with or
without RSO treatment at varying doses (0.125, 0.25, and 0.5 ml/rat/day).
Epilepsy was induced using pilocarpine, and RSO was administered via
intragastric gavage for 28 days prior to seizure induction.
Immunohistochemistry was employed to assess the expression of ZO-1, E-cadherin,
and vimentin in the duodenal mucosa.
Results:
Epilepsy
significantly downregulated the expression of ZO-1, E-cadherin, and vimentin in
the duodenal mucosa, indicating a disruption in both TJs and AJs. RSO treatment
demonstrated a dose-dependent modulation of these proteins. Higher doses of RSO
partially restored ZO-1 and E-cadherin levels in non-epileptic conditions,
though this protective effect was less pronounced in epileptic rats. Vimentin
expression was similarly reduced with epilepsy and high-dose RSO treatment,
suggesting potential destabilization of mesenchymal structures within the
intestinal mucosa.
Conclusion: This study highlights the deleterious effects of
epilepsy on the integrity of intercellular junctions in the duodenal mucosa,
with significant downregulation of key proteins. Rosa Canina Seed Oil
demonstrates promising potential as a therapeutic agent to counteract these
effects, showing that its antioxidant properties can effectively support the
preservation of intestinal barrier function, even in the challenging context of
epilepsy.