A scheme is presented for fractionation of wine components and Fe, Cu and Zn determination in different fractions. The charge of the metal species was established using cation and anion exchange separation based on solid phase extraction. The resin XAD-8 was used for the separation of wine polyphenols in complexes with wine proteins and polysaccharides. Dowex ion exchange resins were used for the separation of cationic and anionic species of Fe, Cu and Zn. Flame atomic absorption spectrometry and electrothermal atomic absorption spectrometry were used off-line for the quantitative determination of metals in the different fractions obtained. (C) 2002 Elsevier Science B.V. All rights reserved.