Protective and antigenotoxic effect of Ulva rigida C. Agardh in experimental hypothyroid.

ÇELİKLER KASIMOĞULLARI S., TAŞ S., Ziyanok-Ayvalik S., VATAN Ö., YILDIZ G., Ozel M.

Acta biologica Hungarica, cilt.65, sa.1, ss.13-26, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65 Sayı: 1
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1556/abiol.65.2014.1.2
  • Dergi Adı: Acta biologica Hungarica
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.13-26
  • Anahtar Kelimeler: Ulva rigida, thyroid, micronucleus, oxidative stress, antigenotoxic effect, volatile compounds, SISTER-CHROMATID EXCHANGE, DIRECT THERMAL-DESORPTION, OXIDATIVE DNA-DAMAGE, LIPID-PEROXIDATION, PERIPHERAL-BLOOD, TAURINE SUPPLEMENTATION, SERUM PARAOXONASE, THYROID-HORMONE, GRAVES-DISEASE, STRESS
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The presence of chromosomal damage in bone marrow cells affected by several diseases such as thyroid, cancer etc., was detected by the micronucleus (MN) assay. The present study was designed to evaluate: i) volatile components of Ulva rigida, ii) effects of hypothyroidism on bone marrow MN frequency, iii) effects of oral administration of Ulva rigida ethanolic extract (URE) on MN frequency produced by hypothyroidism, and iv) thyroid hormone levels in normal and 6-n-Propylthiouracil (PTU)-induced hypothyroid rats. The volatile components of Ulva rigida was studied using a direct thermal desorption (DTD) technique with comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GCxGC-TOF/MS). URE administration was of no significant impact on thyroid hormone levels in control group, while PTU administration decreased thyroid hormone levels compared to control group (p < 0.001). Moreover, URE supplementation resulted in a significant decrease in MN frequency in each thyroid group (p < 0.0001). This is the first in vivo study that shows the strong antigenotoxic and protective effect of URE against the genotoxicity produced by hypothyroidism.