Türkiye Biyolojik Mücadele Dergisi, cilt.13, sa.2, ss.103-117, 2022 (Hakemli Dergi)
The current study was initiated to test the pathogenicity of entomopathogenic fungi against Tuta absoluta. The experiment was conducted at Bursa Uludag University, Horticulture Department glasshouse in 2020/2021. Tuta absoluta adults were collected and larvae were reared on tomatoe seedlings in a growth chamber. Then, ten larvae were treated with each isolate at five inoculum suspension concentrations in a factorial experiment arranged in a completely randomized block design with three replications. The analysis of variance for mortality revealed significant variations (p<0.05) among isolates and concentrations. Metarhizium anisopliae Ak-12 caused the lowest mortality of 80.77% but had the highest LC50 (2.3x108) and the longest incubation period LT50 4.9 and LT90, 9.9 days and considered to be less pathogenic. Whereas Beauveria bassiana Ak-10 showed the highest mortality 91% and the lowest LT50, 4 and LT90, 7.6 days at 1×1010 conidia /ml, followed by Beauveria bassiana Ak-14 and is considered the most aggressive. Conidia concentrations of 1x109 and 1x1010 conidia/ml were the most effective while 1x106 conidia/ml was the least effective. Overall, the current work revealed the potential variation among isolates and concentrations on the mortality of T. absoluta larvae.