Magnetic Poly(VPA-co-EGDMA)-TiO2 Polymeric Microspheres as Supports for Acetylcholinesterase Immobilization: Kinetic Behavior, Stability, and Reusability
Polymers, vol.18, no.16, 2026 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 18 Issue: 16
- Publication Date: 2026
- Doi Number: 10.3390/polym18161999
- Journal Name: Polymers
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, Compendex, INSPEC, Academic Search Ultimate (EBSCO), Engineering Source (EBSCO), Materials Science & Engineering Collection (ProQuest), Technology Collection (ProQuest)
- Keywords: acetylcholinesterase immobilization, enzyme stability and kinetics, multifunctional polymeric microspheres, reusability
- Bursa Uludag University Affiliated: Yes
Abstract
Magnetic poly(vinylphosphonic acid-co-ethylene glycol dimethacrylate)-TiO2 [m-poly(VPA-co-EGDMA)-TiO2] microspheres were synthesized by suspension polymerization and evaluated as supports for direct acetylcholinesterase (AChE) immobilization. The microspheres were characterized by SEM, FT-IR, BET, XRD, XPS, VSM, and zeta potential analyses and exhibited porous morphology, magnetic responsiveness, a specific surface area of 195 m2 g−1, and a swelling ratio of 40.19%. AChE was immobilized mainly through physical adsorption and multipoint non-covalent enzyme–support interactions. The optimum pH was pH 10 for both free and immobilized AChE. Under the tested assay conditions, immobilized AChE showed apparent kinetic parameters of Km = 0.028 mM and Vmax = 0.132 EU.mL−1. Immobilized AChE retained 58.2% of its initial activity after 60 days at 4 °C and 24% after 20 reuse cycles. Overall, the microspheres offer a magnetically recoverable support for AChE immobilization, although further optimization is required to improve kinetic performance and long-term operational stability.