Preventive effects of antenatal CDP-choline in a rat model of neonatal hyperoxia-induced lung injury


Koc C., Cakir A., Salman B., Ocalan B., Alkan T., Kafa İ. M., ...Daha Fazla

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, cilt.101, sa.2, ss.65-73, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 101 Sayı: 2
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1139/cjpp-2022-0321
  • Dergi Adı: CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, SportDiscus, Veterinary Science Database
  • Sayfa Sayıları: ss.65-73
  • Anahtar Kelimeler: betamethasone, bronchopulmonary dysplasia, citicoline, newborn rat, MESSENGER-RNA STABILITY, FETAL, METABOLITES, SURFACTANT, CITICOLINE, GLUCOCORTICOIDS, INVOLVEMENT, ACTIVATION, APOPTOSIS, CELLS
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Antenatal steroid administration to pregnant women at risk of prematurity provides pulmonary maturation in infants, while it has limited effects on incidence of bronchopulmonary dysplasia (BPD), the clinical expression of hyperoxia-induced lung injury (HILI). Cytidine-5'-diphosphate choline (CDP-choline) was shown to alleviate HILI when administered to newborn rats. Therefore, we investigated effects of maternal administration of CDP-choline, alone or in combination with betamethasone, on lung maturation in neonatal rats subjected to HILI immediately after birth. Pregnant rats were randomly assigned to one of the four treatments: saline (1 mL/kg), CDP-choline (300 mg/kg), betamethasone (0.4 mg/kg), or CDP-choline plus betamethasone (combination therapy). From postnatal day 1 to 11, pups born to mothers in the same treatment group were pooled and randomly assigned to either normoxia or hyperoxia group. Biochemical an d histopathological effects of CDP-choline on neonatal lung tissue were evaluated. Antenatal CDP-choline treatment increased levels of phosphatidylcholine and total lung phospholipids, decreased apoptosis, and improved alveolarization. The outcomes were further improved with combination therapy compared to the administration of CDP-choline or betamethasone alone. These results demonstrate that antenatal CDP-choline treatment provides benefit in experimental HILI either alone or more intensively when administered along with a steroid, suggesting a possible utility for CDP-choline against BPD.