Akademik Veri Yönetim Sistemi
14th International Medicine and Heath Sciences Researches Congress, Ankara, Türkiye, 23 - 24 Aralık 2023, cilt.14, ss.378-379
The Leptin gene, alternatively recognized as the obesity gene, influences food consumption, energy balance, and the lipid metabolism. It also holds a significant position in energy metabolism and is situated on the 4th chromosome of the goat genome. Polymorphisms in the Leptin gene have been investigated across diverse farm animals, revealing associations with carcass fatty acid composition, as well as traits related to carcass and meat quality. Additionally, these gene variations are linked to aspects such as milk yield, milk fat content, milk protein, and the composition of milk fatty acids. In the regions investigated in this study, a noteworthy association was identified between exon 2 and carcass traits in both cattle and pigs. Additionally, a significant relationship with production traits in cattle was reported for the intron 2 region. Yet, there is a restricted understanding of these genetic variants in goats at present. Therefore, this study aimed to determine the polymorphisms in the intron 2 and exon 2 regions of the LEP gene in Saanen goats. Genomic DNA was extracted from blood samples collected from a total of 103 goats using the Thermo Scientific GeneJET Genomic DNA Purification Kit. Genotyping was carried out by the PCR-RFLP method. The AB genotype exhibits the highest frequency for the variant located in intron 2. Allele frequencies were calculated as 0.641 and 0.359 for A and B, respectively. The variation in this polymorphism was compatible with Hardy-Weinberg equilibrium. On the other hand, the polymorphism in the exon 2 region is monomorphic for the population examined. The findings of this study will offer valuable preliminary insights for future investigations into the Leptin gene in goats.