Purification and crystallization of alpha-amylases from mucoid and non-mucoid B-amyloliquefaciens strains


Sarikaya E. , Mikami B.

JOURNAL OF CRYSTAL GROWTH, vol.232, pp.418-420, 2001 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 232
  • Publication Date: 2001
  • Doi Number: 10.1016/s0022-0248(01)01088-0
  • Title of Journal : JOURNAL OF CRYSTAL GROWTH
  • Page Numbers: pp.418-420

Abstract

The purified alpha -amylases from mucoid and non-mucoid B. amyloliquefaciens strains were crystallized in forms suitable for X-ray diffraction analysis. Crystals were grown by the hanging-drop vapor diffusion method. Very thin crystals of alpha -amylase of non-mucoid strain were obtained in the presence 15% propanol, 12% PEG 6000 and 0.1 M PIPES (pH: 7.1) at the end of 3 months and these were not suitable for X-ray diffraction analysis. Crystals of the mucoid strain of B. amyloliquefaciens alpha -amylase were obtained with 30% PEG 6000, 0.2 M (NH4)(2)SO4 and 0.1 M PIPES (pH: 6.5) at the end of 3 months and these were suitable for X-ray diffraction analysis. (C) 2001 Elsevier Science B.V. All rights reserved.