Purification and crystallization of alpha-amylases from mucoid and non-mucoid B-amyloliquefaciens strains

Sarikaya E., Mikami B.

JOURNAL OF CRYSTAL GROWTH, cilt.232, ss.418-420, 2001 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 232
  • Basım Tarihi: 2001
  • Doi Numarası: 10.1016/s0022-0248(01)01088-0
  • Dergi Adı: JOURNAL OF CRYSTAL GROWTH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.418-420
  • Bursa Uludağ Üniversitesi Adresli: Hayır

Özet

The purified alpha -amylases from mucoid and non-mucoid B. amyloliquefaciens strains were crystallized in forms suitable for X-ray diffraction analysis. Crystals were grown by the hanging-drop vapor diffusion method. Very thin crystals of alpha -amylase of non-mucoid strain were obtained in the presence 15% propanol, 12% PEG 6000 and 0.1 M PIPES (pH: 7.1) at the end of 3 months and these were not suitable for X-ray diffraction analysis. Crystals of the mucoid strain of B. amyloliquefaciens alpha -amylase were obtained with 30% PEG 6000, 0.2 M (NH4)(2)SO4 and 0.1 M PIPES (pH: 6.5) at the end of 3 months and these were suitable for X-ray diffraction analysis. (C) 2001 Elsevier Science B.V. All rights reserved.