REVUE DE MEDECINE VETERINAIRE, cilt.154, sa.7, ss.459-462, 2003 (SCI-Expanded)
In the present study, ten ejaculates were obtained from each of seven German shepherd dogs. Only the sperm-rich fractions of ejaculates from each dog once a week with a mean interval of 60 min, were used during five weeks. Semen quality was examined in total of 70 semen samples. After this examination, semen samples were centrifuged (5000 g x 10 minutes) and seminal plasma were separated. Plasma total protein, calcium, phosphorus, magnesium, sodium and potassium and alkaline phosphatase were determined. The semen quality and biochemical findings were compared between the first and the second ejaculate following very-short interval ejaculation.