The aim of this study was to characterize the bovine fatty acid binding protein (FABP4) gene 3691G > A and 2834C > G polymorphisms and to evaluate interaction effects on the live weight, carcass characteristics and meat quality of Holstein bulls in the South Marmara region of Turkey. A total of 400 Holstein bulls grown on a private farm and slaughtered at 14-21 months of age, were randomly selected for use in this study. Initially, genotyping was performed by PCR-RFLP method and statistical analysis was carried out using least square methods of the GLM procedure. A SNP located in intron 1 (2834C > G) was associated with desirable increases in live weight, hot carcass weight and chilled carcass weight, and a SNP located in exon 3 (3691G > A) was associated with significant increases in marbling scores and first compressive stress point of the longissimus dorsi muscle (P < 0.05). The interaction analysis of the 3691G > A and the 2834C > G polymorphisms revealed significant effects for hot carcass weight, chilled carcass weight and backfat thickness (P < 0.05). There were no significant associations between the SNPs and carcass measurements. Results indicated that the FABP4 3691G > A and 2834C > G polymorphisms and the 3691G > A and 2834C > G interactions can be used as selection parameters in breeding programmes to improve meat yield and carcass characteristics.