© 2021 Wolters Kluwer Medknow Publications. All rights reserved.Aim: This study aims to comparatively evaluate the flexural strength, internal adaptation, elastic modulus, and maximum deflection of a newly introduced, strengthened injection-molded semi-flexed polyamide resin (Deflex) and a conventional heat-cured resin containing cross-linking polymethyl methacrylate denture base polymers (QC-20). Materials and Methods: A vinyl polysiloxane film replicating the gap between the denture base and the metallic master model of an edentulous maxilla was weighed using an analytical balance with an accuracy of 0.0001 g for the measurement of internal adaptation. The measurements were performed immediately after surface finishing. Seven rectangular test samples measuring 65 × 10 × 3.3 mm 3 were set up for flexural strength test. Flexural strength test (three-point bending test) was performed using a universal machine under axial load at a crosshead speed of 5 mm/min. One-way ANOVA (α = 0.05) following by t tests was utilized in statistical analysis. Results: The difference between the flexural strength of the denture base resins of Deflex and QC-20 was found to be statistically significant. The injection-molded resin demonstrated better internal adaptation compared to the conventional heat-polymerized resin. Evaluation of the physical test results revealed that the polyamide samples were more flexible than polymethyl methacrylate and did not break during flexural strength tests. Conclusion: Some properties of denture base resins, such as resin types, internal adaptation, and mechanical strength, may play a significant role in clinical performance of complete dentures and removable partial prostheses. Because of the superior flexural strength properties and internal adaptation characteristics, Deflex may prove to be a useful alternative to conventional denture base resin.