Surface imprinted bacterial cellulose nanofibers for hemoglobin purification


Bakhshpour M., Tamahkar E., ANDAÇ A. M. , DENİZLİ A.

COLLOIDS AND SURFACES B-BIOINTERFACES, vol.158, pp.453-459, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 158
  • Publication Date: 2017
  • Doi Number: 10.1016/j.colsurfb.2017.07.023
  • Journal Name: COLLOIDS AND SURFACES B-BIOINTERFACES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.453-459
  • Keywords: Hemoglobin purification, Surface imprinting, Metal ion coordination, Bacterial cellulose nanofibers, METAL COORDINATION, BOVINE HEMOGLOBIN, ALBUMIN DEPLETION, RECOGNITION, POLYMER, NANOPARTICLES, COMPOSITE, AFFINITY, CRYOGEL, SEPARATION
  • Bursa Uludag University Affiliated: No

Abstract

There is a significant need for the development of the novel adsorbents in the field of protein purification. In this study, thin hemoglobin imprinted film (MIP) was fabricated onto the bacterial cellulose nanofibers' (BCNFs) by surface imprinting method using metal ion coordination interactions with N-methacryloyl-(L)-histidinemethylester (MAH) and copper ions. The hemoglobin surface imprinted bacterial cellulose nanofibers (MIP-BCNFs) was applied to selective recognition of hemoglobin and purification from hemolysate. The characterization of the MIP-BCNFs was carried out by the Fourier Transformed Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), micro-Computerized Tomography (mu CT), atomic force microscopy (AFM) and surface area measurements. The adsorption experiments of hemoglobin onto the MIP-BCNFs and NIP-BCNFs from aqueous hemoglobin solutions were investigated in a batch system. The results showed that MIP-BCNFs are promising materials for purification of hemoglobin with high adsorption capacity, significant selectivity and reusability. (C) 2017 Elsevier B.V. All rights reserved.