Rapid, practical and safe isolation of adipose derived stem cells

Cerci E., ERDOST H.

BIOTECHNIC & HISTOCHEMISTRY, vol.96, no.2, pp.138-145, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 96 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.1080/10520295.2020.1776895
  • Journal Name: BIOTECHNIC & HISTOCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.138-145
  • Keywords: Adipose tissue, culture flasks, isolation, mesenchymal stem cell, rat, scratched flask
  • Bursa Uludag University Affiliated: Yes

Abstract

We compared migration, proliferation, growth curve, confluency and differentiation into adipogenic, osteogenic and chondrogenic cell lineages of mesenchymal stem cells derived from adipose tissue cultured in scratched and nonscratched cell culture flasks. Mesenchymal stem cells were isolated from rat adipose tissue using a nonenzymatic method. We investigated two groups. For the control group, minced adipose tissue was implanted conventionally onto the surface of standard plastic cell culture flasks. For the experimental group, the tissues were cultured in flasks with a scratched surface. We found that scratched flasks promoted cell migration, proliferation and confluency. Our findings suggest that scratched flasks may be used to ensure rapid, practical and safe isolation of adipose tissue-derived stem cells.