The effect of centrally injected CDP-choline on respiratory system; involvement of phospholipase to thromboxane signaling pathway

Topuz B. B., Altinbas B., Yilmaz M. S., Saha S., Batten T. F., SAVCI V., ...More

Respiratory Physiology and Neurobiology, vol.195, no.1, pp.50-58, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 195 Issue: 1
  • Publication Date: 2014
  • Doi Number: 10.1016/j.resp.2014.02.005
  • Journal Name: Respiratory Physiology and Neurobiology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.50-58
  • Keywords: CDP-choline, Intracerebroventricular, Respiratory frequency, Tidal volume, Minute ventilation, Central prostaglandinergic system, Central cholinergic receptors, INCREASES PLASMA VASOPRESSIN, ARACHIDONIC-ACID, BRAIN, RAT, PRESSOR, ACTIVATION, A(2), HYPOTENSION, CYTIDINE, RECEPTOR
  • Bursa Uludag University Affiliated: Yes

Abstract

CDP-choline is an endogenous metabolite in phosphatidylcholine biosynthesis. Exogenous administration of CDP-choline has been shown to affect brain metabolism and to exhibit cardiovascular, neuroendocrine neuroprotective actions. On the other hand, little is known regarding its respiratory actions and/or central mechanism of its respiratory effect. Therefore the current study was designed to investigate the possible effects of centrally injected CDP-choline on respiratory system and the mediation of the central cholinergic receptors and phospholipase to thromboxane signaling pathway on CDP-choline-induced respiratory effects in anaesthetized rats.Intracerebroventricularly (i.c.v.) administration of CDP-choline induced dose- and time-dependent increased respiratory rates, tidal volume and minute ventilation of male anaesthetized Spraque Dawley rats. İ.c.v. pretreatment with atropine failed to alter the hyperventilation responses to CDP-choline whereas mecamylamine, cholinergic nicotinic receptor antagonist, mepacrine, phospholipase A2 inhibitor, and neomycin phospholipase C inhibitor, blocked completely the hyperventilation induced by CDP-choline. In addition, central pretreatment with furegrelate, thromboxane A2 synthesis inhibitor, also partially blocked CDP-choline-evoked hyperventilation effects.These data show that centrally administered CDP-choline induces hyperventilation which is mediated by activation of central nicotinic receptors and phospholipase to thromboxane signaling pathway. © 2014 Elsevier B.V.