Anticancer Potential of Albumin Bound Wnt/beta-Catenin Pathway Inhibitor Niclosamide in Breast Cancer Cells

Arı F., Erkisa M., Pekel G., Ertürk E., Buyukkoroglu G., Ulukaya E.

CHEMISTRYSELECT, vol.6, no.29, pp.7463-7475, 2021 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 29
  • Publication Date: 2021
  • Doi Number: 10.1002/slct.202100819
  • Journal Name: CHEMISTRYSELECT
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier
  • Page Numbers: pp.7463-7475
  • Keywords: Albumin, Apoptosis, Breast cancer, Nanoparticles, Niclosamide, DRUG-DELIVERY SYSTEM, GELATIN NANOPARTICLES, OPTIMIZATION
  • Bursa Uludag University Affiliated: Yes

Abstract

Albumin-based nanoparticle transport systems (nab-technology) are a new strategy in cancer treatment and we aimed to increase the effectiveness of Niclosamide using this technology. Niclosamide was bound with bovine serum albumin (BSA) by desolvation to yield nanoparticle albumin-bound Niclosamide (nab-Niclo). Nab-Niclo anticancer activity was assessed by proliferation, apoptosis and DNA damage analyses on breast cancer cells. The results implied that nab-Niclo was a more potent agent in the inhibition of cell viability than free Niclosamide and albumin. Flow cytometry analysis show that nab-Niclo triggered apoptosis by caspase and mitochondriadependent pathways in cells and nab-Niclo enhances apoptosis by induce DNA damage in cells. Overall results of this study showed that the nanoparticle form of Niclosamide is effective for breast cancer treatment, presenting a new treatment strategy that can be safe and effective for breast cancer patients.