Experimental subarachnoid haemorrhage models in rats

Alkan T., Korfali E., Kahveci N.

Conference on Research and Publishing Neurosurgery, Antalya, Türkiye, 01 Ocak 2001, cilt.83, ss.61-69 identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası: 83
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.61-69
  • Anahtar Kelimeler: acute vasoconstriction, cerebral blood flow, animal models, intracranial pressure, subarachnoid haemorrhage, CEREBRAL BLOOD-FLOW, CANINE CAROTID ARTERIES, IN-VIVO ANGIOPLASTY, BASILAR ARTERY, PRIMATE MODEL, ACUTE VASOCONSTRICTION, MORPHOLOGICAL ANALYSES, ANIMAL-MODEL, NITRIC-OXIDE, HEMORRHAGE
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

There is no comprehensive and reliable model available in small animals that are suitable for the study of subarachnoid haemorrhage (SAH). In the study we reviewed the advantages and disadvantages of available SAH models in rats and presented our model. Experimental SAH was induced in a group of 350-450 g SpragueDawley rats. A 2 mm-diameter burr hole was drilled and, working under a microscope, haemorrhage was produced by transclival puncture of the basilar artery with a 20 mum thick piece of glass. The rats were assigned to either the experimental group (n: 7) or the control group (n: 7). Local cerebral blood flow (LCBF), intracranial pressure (ICP), and cerebral perfusion pressure (CPP) were measured for 60 min after SAH, after which the rats were decapitated. Microscopic examinations were done on three different segments of the basilar artery.