Experimental subarachnoid haemorrhage models in rats


Alkan T., Korfali E., Kahveci N.

Conference on Research and Publishing Neurosurgery, Antalya, Turkey, 01 January 2001, vol.83, pp.61-69 identifier

  • Publication Type: Conference Paper / Full Text
  • Volume: 83
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.61-69
  • Bursa Uludag University Affiliated: Yes

Abstract

There is no comprehensive and reliable model available in small animals that are suitable for the study of subarachnoid haemorrhage (SAH). In the study we reviewed the advantages and disadvantages of available SAH models in rats and presented our model. Experimental SAH was induced in a group of 350-450 g SpragueDawley rats. A 2 mm-diameter burr hole was drilled and, working under a microscope, haemorrhage was produced by transclival puncture of the basilar artery with a 20 mum thick piece of glass. The rats were assigned to either the experimental group (n: 7) or the control group (n: 7). Local cerebral blood flow (LCBF), intracranial pressure (ICP), and cerebral perfusion pressure (CPP) were measured for 60 min after SAH, after which the rats were decapitated. Microscopic examinations were done on three different segments of the basilar artery.