Application of affinity microspheres for effective SPE cleanup before the determination of sulfamerazine by HPLC

Kilic G., OSMAN B., Tuzmen N.

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, cilt.91, ss.55-63, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 91
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.msec.2018.04.058
  • Dergi Adı: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.55-63
  • Anahtar Kelimeler: Affinity microspheres, Antibiotic, Sulfamerazine, SPE cleanup, HPLC, SOLID-PHASE EXTRACTION, PERFORMANCE LIQUID-CHROMATOGRAPHY, PERSONAL CARE PRODUCTS, AQUEOUS-SOLUTION, MASS-SPECTROMETRY, MALACHITE-GREEN, ESTROGENIC CHEMICALS, ANTIBIOTIC-RESIDUES, CONFIRMATORY METHOD, ACTIVATED CARBON
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

This paper describes the application of poly (ethylene glycol dimethacrylate-N-methacryloyl-L-tryptophane methyl ester) [p(EGDMA-MATrp)] microspheres as an affinity sorbent for the SPE (solid phase extraction) cleanup of sulfamerazine (SMR) from food samples of animal origin before high performance liquid chromatography (HPLC) analysis. The microspheres were prepared by suspension polymerization of ethylene glycol dimethacrylate (EGDMA) and N-methacryloyl-L-tryptophane methyl ester (MATrp) as a crosslinker and a monomer, respectively. Various parameters affecting the SPE cleanup efficiency of the p(EGDMA-MATrp) microspheres (contact time, pH, initial SMR concentration, ionic strength etc.) were optimized. Under the optimized conditions, maximum adsorption capacity was found to be 8.55 +/- 0.44 mg/g sorbent at pH 5.0. 90% of the adsorbed SMR was desorbed by using ACN:MeOH (5:5) mixture as a desorption agent. Applicability of the microspheres for the SPE cleanup of SMR residues in the food samples such as egg and milk with HPLC was also determined. It was demonstrated that the prepared p(EGDMA-MATrp) microspheres could be repeatedly applied for SPE cleanup of sulfamerazine before chromatographic analysis. Also, the recoveries of SMR in milk and egg samples were reasonably satisfactory and in the range of 71 to 90%.