Association of the caprine calpastatin MspI polymorphism with growth and reproduction traits in Saanen goats

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Ardıçlı S., Üstüner H., Arslan Ö.

Journal of the Hellenic Veterinary Medical Society, vol.72, no.3, pp.3023-3030, 2021 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 72 Issue: 3
  • Publication Date: 2021
  • Doi Number: 10.12681/jhvms.28483
  • Journal Name: Journal of the Hellenic Veterinary Medical Society
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, Veterinary Science Database
  • Page Numbers: pp.3023-3030
  • Bursa Uludag University Affiliated: Yes


This study was designed to evaluate the effects of calpastatin (CAST) MspI polymorphism on some

growth and reproduction traits, including birth weight, first breeding weight, litter size, and average daily weight gain

in Saanen goats. In this sense, blood samples obtained from 73 purebred female Saanen goats were used for genotyping.

Genomic DNA was isolated by the phenol-chloroform method and used to determine CAST genotypes, including

MM, MN, and NN, by means of the PCR-RFLP method. The population genetic parameters were estimated based on

allelic distribution and the data were statistically analysed using analysis of variance (ANOVA) using a general linear

model (GLM). Results revealed that N allele frequency was remarkably high (0.64) and the MM genotype was not

present. The frequency of the heterozygous genotype was 59.62%. Concerning ANOVA results, significant differences

were found between genotypes of the CAST locus concerning birth weight (P<0.05). In this respect, animals with

the NN genotype were associated with higher birth weight means (2.85±0.29 kg) compared to heterozygous animals

(2.53±0.24 kg). There was no significant association between the CAST marker and any of the remaining phenotypic

traits evaluated. The present results suggest that focusing on this genomic region may be particularly useful in improving

birth weight in goats which can be considered as an early indicator of post-weaning animal growth and survival.