A portable microfluidic platform for rapid determination of microbial load and somatic cell count in milk


Duven G., Cetin B., Kurtuldu H., Gunduz G. T. , Tavman Ş., Kışla D.

BIOMEDICAL MICRODEVICES, vol.21, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 21
  • Publication Date: 2019
  • Doi Number: 10.1007/s10544-019-0407-8
  • Journal Name: BIOMEDICAL MICRODEVICES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Microfluidics, Quality of milk, Somatic cell count, Bacteria count, LISTERIA-MONOCYTOGENES, PATHOGEN DETECTION, FLOW-CYTOMETRY, BACTERIA, CHIP, IDENTIFICATION, SEPARATION, DEVICE
  • Bursa Uludag University Affiliated: Yes

Abstract

Microfluidics systems that have been emerged in the last 20years and used for processing the fluid in a microchannel structure at microliter levels are alternative to the conventional methods. The objective of the study is to develop a microfluidic platform for determination of the microbial load and the number of somatic cells in milk. For this purpose, a polydimethylsiloxane (PDMS) chip with a channel size of 300mx60m was produced. Cells/bacteria labeled with fluorescent stain in milk were counted with the proposed microfluidic platform and the results were compared with the reference cell concentration/the bacterial counts by conventional method. It was found that our platform could count somatic and bacterial cells with an accuracy above 80% in 20min run for each analysis. The portable overall platform has an overall dimension of 25x25x25 cm and weighs approximately 9kg.