Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders

ALÇAY S., Cakmak S., ÇAKMAK İ., Mulkpinar E., GÖKÇE E., ÜSTÜNER B., ...Daha Fazla

CRYOBIOLOGY, cilt.87, ss.28-31, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 87
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.cryobiol.2019.03.005
  • Dergi Adı: CRYOBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.28-31
  • Anahtar Kelimeler: Honey bee, Apis mellifera, Royal jelly, Drone semen, Cryopreservation, POSTTHAW QUALITY, RAM SEMEN, ANTIOXIDANT, QUEENS, PLASMA
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thawing quality of drone sperm. Semen samples were collected from sexually mature drones. Pooled semen was diluted with extender without RJ (control) or supplemented with different concentrations of RJ (1, 2, 4 or 8%). Sperm motility, plasma membrane functional integrity, and acrosomal integrity were evaluated. At post thaw, the highest sperm motility and acrosomal integrity rates were obtained in the RJ1 group. Functional integrity of sperm membrane was better preserved in the RJ1 and RJ2 groups compare to the other groups. The study shows that RJ supplemented extenders have beneficial effects on drone semen parameters. The results of the present study demonstrated advantage of using 1% RJ supplemented extender.