İzotermal Amplifikasyon Yöntemleri ve Virolojideki Kullanım Alanları


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Toker E. B. , Aytoğu G., Yeşilbağ K.

3rd International Eurasian Conference onBiological and Chemical Sciences(EurasianBioChem 2020), Ankara, Turkey, 19 - 20 March 2020, pp.163-173

  • Publication Type: Conference Paper / Full Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.163-173
  • Bursa Uludag University Affiliated: Yes

Abstract

Nucleic acid amplification methods based on the detection and analysis of nucleic acid are frequently used in the forensic medicine and diagnosis of infectious, genetic and tumoral diseases. One of these methods, polymerase chain reaction (PCR), is the most widely used DNA amplification method. In the PCR methods, there is required extensive and high cost laboratory equipment and a thermalcycler. Nowadays, some new techniques that allow the amplification of DNA under isothermal conditions without the need for a thermalcycler have been developed. These techniques include transcription mediated amplification (TMA), self-sustained sequence replication (3SR), nucleic acid sequence-based amplification (NASBA), strand displacement amplification (SDA), loop-mediated isothermal amplification (LAMP), Recombinase polimerase amplification (RPA) and helicase-dependent amplification (HDA) are among the most widely used methods in the field of virology. In this rewiew paper, it is aimed to explain the basic principles of these isothermal nucleic acid amplification methods and opportunities for field applications in virological researchs.