Validation and characterization of murine gammaherpesvirus 68 antisense transcripts by northern blot analysis and quantitative reverse transcription-PCR

Kara M.

ARCHIVES OF BIOLOGICAL SCIENCES, cilt.75, sa.2, ss.16, 2023 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 75 Sayı: 2
  • Basım Tarihi: 2023
  • Doi Numarası: 10.2298/abs230407016k
  • Dergi Adı: ARCHIVES OF BIOLOGICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.16
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The transcription of mammalian genomes exhibits an intriguing complexity and numerous novel RNA molecules have been identified. Viruses with large DNA genomes, especially herpesviruses, generate many different RNA species, including long non-coding RNAs (lncRNAs). Dense viral genomes can generate multigenic transcripts in addition to commonly observed antisense transcripts. It is essential to study the biological roles of these transcripts aside from the protein-coding counterparts. Multiple antisense transcripts from the open reading frame (ORF) 63-64 locus in murine gammaherpesvirus 68 (MHV68) were detected by northern blotting. Expression analysis by quantitative reverse transcription PCR (qRT-PCR) did not detect different isoforms. Several alternative splicing isoforms exist during lytic replication; however, they are not detected during latency. To identify the roles of these new transcripts, qRT-PCR may not be enough and should be supported by an alternative method such as northern blotting. A more detailed transcriptional map of the locus of interest is useful to design experimental strategies and perform functional studies, especially when working with genedense viral genomes.