Brain thromboxane A(2) via arachidonic acid cascade induces the hypothalamic-pituitary-gonadal axis activation in rats


Erkan L. G. , Altinbas B., Guvenc G., ALÇAY S., Toker M. B. , ÜSTÜNER B., ...More

AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL, vol.189, pp.50-55, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 189
  • Publication Date: 2015
  • Doi Number: 10.1016/j.autneu.2015.02.005
  • Journal Name: AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.50-55
  • Keywords: Arachidonic acid, Cyclooxygenase, Thromboxane A(2), Hypothalamic-pituitary-gonadal axis, Sperm parameters, Intracerebroventricular, FOLLICLE-STIMULATING-HORMONE, PROTEIN-KINASE-C, LUTEINIZING-HORMONE, GONADOTROPIN-SECRETION, INSULIN-SECRETION, LIPOXYGENASE PRODUCTS, PROLACTIN-RELEASE, HYPOTENSIVE RATS, BLOOD-PRESSURE, SPERM QUALITY
  • Bursa Uludag University Affiliated: Yes

Abstract

The current study was designed to determine the effect of centrally administrated arachidonic acid (AA) on plasma gonadotropin hormone-releasing hormone (GnRH), follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone level, and sperm parameters, and to show the mediation of the central cyclooxygenase (COX) to thromboxane A(2) (TXA(2)) signaling pathway in AA-induced hormonal and sperm parameter effects. Studies were performed in male Sprague-Dawley rats. A total of 150 or 300 mu l/5 mu l doses of AA were injected intracerebroventricularly (icv). AA significantly caused dose- and time-dependent increases in plasma FSH, LH and testosterone levels of animals, but not plasma GnRH level. AA also significantly increased sperm motility of the rats without change sperm number. Pretreated with ibuprofen, a nonselective COX inhibitor (250 mu g/5 mu l; icv), and furegrelate, a TXA(2) synthesis inhibitor (250 mu g/5 mu l; icv), prevented AA-evoked increase in plasma FSH, LH and testosterone levels, and sperm motility.