Evaluation of Nigella sativa oil loaded electrospun polyurethane nanofibrous mat as wound dressing

Aras C., Ozer E., Göktalay G., Saat G., Karaca E.

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, vol.32, pp.1718-1735, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 32
  • Publication Date: 2021
  • Doi Number: 10.1080/09205063.2021.1937463
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, EMBASE, INSPEC, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.1718-1735
  • Keywords: Nigella sativa oil, polyurethane, nanofiber, wound dressing, release, wound healing, DRUG-DELIVERY, THERAPEUTIC AGENTS, POLYMER NANOFIBERS, ACTIVE PRINCIPLE, RELEASE SYSTEMS, THYMOQUINONE
  • Bursa Uludag University Affiliated: Yes


Electrospun nanofibers have a natural wound healing effect due to their similarity to the extracellular matrix (ECM). Nigella sativa oil, which has therapeutic properties, is used for a wide variety of applications in traditional medicine. The aim of this study was to investigate the release characteristic and wound healing performance of Nigella sativa oil (NSO) loaded polyurethane (PU) electrospun nanofibrous mats in wound dressing applications. In addition, the antibacterial activity and cytotoxicity of the electrospun mats were studied. Analyses using a scanning electron microscope (SEM) showed that PU/NSO nanofibrous mat with an average fiber diameter of 416 +/- 66 nm were successfully fabricated. NSO was released at a maximum ratio of 30% from the electrospun mat, and the Korsmeyer-Peppas model was identified as best for determining the release mechanism. Significant antibacterial activity was observed against Staphylococcus aureus (90.26%) and Escherichia coli (95.75%). The developed PU/NSO nanofibrous mat increased the cell viability more than 100% in human umbilical vein endothelial cell line (HUVEC) cell line. The NSO loaded PU nanofibrous mat significantly promoted the wound healing process on a rat wound model, and its wound closure reached approximately 85% compared to the control groups on the 9(th) day (p < 0.01). The results indicated PU/NSO nanofibrous mat is a suitable candidate for a wound dressing.