Grape seed extract supplementation in heat-stressed preweaning dairy calves: I. Effects on antioxidant status, inflammatory response, hematological and physiological parameters


Biricik H.

ANIMAL FEED SCIENCE AND TECHNOLOGY, vol.292, pp.115421-115431, 2022 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 292
  • Publication Date: 2022
  • Doi Number: 10.1016/j.anifeedsci.2022.115421
  • Journal Name: ANIMAL FEED SCIENCE AND TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Food Science & Technology Abstracts, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.115421-115431
  • Bursa Uludag University Affiliated: Yes

Abstract

This study aimed to investigate the effects of grape seed extract (GSE) supplementation on

antioxidant activity, inflammatory markers, hematological and physiological responses during

the suckling period in heat-stressed calves. Sixty Holstein female calves (3 d of age, 40.6 ± 2.17

kg of BW) were randomly divided into four dietary groups. Each group consisted of 15 calves; the

milk was supplemented with 0 (CON) 25 (GSE1), 50 (GSE2), and 100 (GSE3) mg/kg BW/day of

GSE. On d 3, 33, and 63 blood samples were collected, rectal temperatures (RT) and respiratory

rates (RR) were measured three days a week. Calves were exposed to chronic heat stress during

the study period and were weaned on d 63. Plasma malondialdehyde (MDA) and tumor necrosis

factor-alpha (TNF-α) concentrations decreased (P < 0.01) in all GSE supplemented groups

compared to the CON group. Also, interferon-gamma (IFN-γ) concentration decreased (P = 0.03)

only in calves fed GSE1. Plasma total antioxidant capacity (TAC) concentration increased (P =

0.02) in calves fed GSE3 on d 33 compared to the CON group, while the concentration of superoxide

dismutase (SOD) was higher (P < 0.001) in the GSE2 and GSE3 groups compared to CON

and GSE1. The hematocrit percentage, hemoglobin value, RBC, and eosinophil counts were higher

(P < 0.01) in all GSE supplemented groups compared to CON. Calves fed GSE1, GSE2 and GSE3

had lower (P < 0.001) neutrophil count compared to CON on d 63. All GSE-supplemented groups

had a decreased (P < 0.001) RR compared to CON, while calves fed GSE2 had a tendency to

decrease (P = 0.10) in RT. It was concluded that supplementation with 50 and 100 mg/kg BW/

d GSE could improve antioxidant, anti-inflammatory activities, and hematological parameters of

calves against oxidative stress, and also reduce the effect of heat stress by reducing the respiratory

rate.