THE CLASTOGENIC EFFECT OF PYRIMETHAMINE (DARAPRIM) ON HUMAN-CHROMOSOMES IN LYMPHOCYTE-CULTURES

EGELI Ü., ERDOGAN G.

CELL BIOLOGY AND TOXICOLOGY, vol.7, no.4, pp.347-356, 1991 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 4
  • Publication Date: 1991
  • Doi Number: 10.1007/bf00124070
  • Journal Name: CELL BIOLOGY AND TOXICOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.347-356
  • Bursa Uludag University Affiliated: Yes

Abstract

In this study the clastogenic effect of pyrimethamine (Daraprim), a folic acid antagonist used for the treatment of toxoplasmosis and malaria on human chromosomes, was investigated. Pyrimethamine was added to in vitro lymphocyte cultures at six different concentrations: 0.05 (normal therapeutic dose), 0.1, 0.2, 0.4, 0.8, and 1.6 mg/ml. No proliferation was observed in any of the cultures containing 1.6 mg/ml pyrimethamine. The results of the cytogenetic evaluations show that the frequency of breaks and gaps increase significantly in dose-dependent manner. Thus, pyrimethamine has a clastogenic effect on human chromosomes.