Choline increases serum insulin in rat when injected intraperitoneally and augments basal and stimulated aceylcholine release from the rat minced pancreas in vitro

Ilcol Y., Gurun M. S. , Taga Y., Ulus I.

EUROPEAN JOURNAL OF BIOCHEMISTRY, vol.270, no.5, pp.991-999, 2003 (SCI-Expanded) identifier identifier identifier


Intraperitoneal injection of choline (30-90 ) produced a dose-dependent increase in serum insulin, glucose and choline levels in rats. The increase in serum insulin induced by choline (90 ) was blocked by pretreatment with the muscarinic acetylcholine receptor antagonists, atropine (2 ), pirenzepine (2 ) and 4-diphenylacetoxy-N -methylpiperidine (2 ) or the ganglionic nicotinic receptor antagonist, hexamethonium (15 ). The effect of choline on serum insulin and glucose was enhanced by oral glucose administration (3 ). Choline administration was associated with a significant (P < 0.001) increase in the acetylcholine content of pancreatic tissue. Choline (10-130 mum) increased basal and stimulated acetylcholine release but failed to evoke insulin release from the minced pancreas at considerably higher concentrations (0.1-10 mm). Hemicholium-3, a choline uptake inhibitor, attenuated the increase in acetylcholine release induced by choline augmentation. Choline (1-32 mm) inhibited [(3) H]quinuclidinyl benzilate binding to the muscarinic receptors in the pancreatic homogenates. These data show that choline, a precursor of the neurotransmitter acetylcholine, increases serum insulin by indirectly stimulating peripheral acetylcholine receptors through the enhancement of acetylcholine synthesis and release.