5th International Molecular Immunology & Immunogenetics Congress, İzmir, Turkey, 20 - 22 October 2022, pp.103
Objective: Investigation the effect of the storage time of red blood cell concentrates (RBC) on the T lymphocytes viability and proliferation ability. Materials-Methods: Two units of whole blood (WB) were donated by volunteers. Also, a blood sample was taken from each volunteer before donation. RBCs were obtained from WBs and divided into five equal parts for the preparation of samples for 0, 7, 14, 21, 42 storage days (D0, D7, D14, D21, D42). Mononuclear cells (MNC) were isolated from RBCs and donor blood samples by the density gradient method (Ficoll 1077). Viability and proliferation analyses of T cell (CD3, CD4, CD8) were performed using PI and CFSE, respectively via a flow-cytometer. Additionally, the activation markers (CD25, CD69, CD154) were analysed in the PI-negative groups. All tests mentioned above were performed until D21. Tests about D42 could not be carried out due to their storage time didn’t completed. Also, white blood cells (WBC) and MNCs were isolated from a volunteer by the density gradient method (Ficoll 1119 and 1077, respectively) for MTT tests. MTT analyses were carried out to investigate the effect of additive solutions (which exist in the RBCs), erythrocytes and phytohemagglutinin on the viability of these WBCs and MNCs. Results: The most increased T cell viability was detected in blood samples taken before donation while the lowest level was seen in D0 samples. We observed that viability ascended during storage time compared to D0. The highest proliferation capability of T cells was detected in D14 samples. We determined that proliferation ability improved until D14, then it diminished to their lowest level in D21. Conclusions: This study indicates that the viability of T cells in RBCs increases during storage time and their proliferation skills which are increased for a certain period then dramatically decreases.