Vinylferrocene copolymers based biosensors for phenol derivatives

DURSUN F., Ozoner S. K., Demirci A., Gorur M., Yilmaz F., Erhan E.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.87, no.1, pp.95-104, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 87 Issue: 1
  • Publication Date: 2012
  • Doi Number: 10.1002/jctb.2688
  • Journal Name: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.95-104
  • Keywords: vinylferrocene, glycidyl methacrylate, 3-methylthienyl methacrylate, conductive composites, immobilized tyrosinase, redox polymers, AMPEROMETRIC ENZYME ELECTRODE, HORSERADISH-PEROXIDASE, CONDUCTING POLYMERS, 3-METHYLTHIENYL METHACRYLATE, POLY(VINYLFERROCENE-CO-2-HYDROXYETHYL METHACRYLATE), COVALENT IMMOBILIZATION, GLUCOSE-OXIDASE, FERROCENE, FILM, MEDIATOR
  • Bursa Uludag University Affiliated: No

Abstract

BACKGROUND: Newly synthesized composite films of P(glycidyl methacrylate85-co-vinylferrocene15)/Poly(glutaraldehyde)/ Polypyrrole [P(GMA85-co-VFc15)/PGA/PPy] and Poly(3-methylthienyl methacrylate85-co-vinylferrocene15)/Polypyrrole [P (MTM85-co-VFc15)/PPy] were used as matrices for tyrosinase based working electrodes. Direct covalent attachment of enzyme was carried out via the pendant epoxy groups of P(GMA85-co-VFc15) film, and the entrapment of enzyme was achieved for electrode containing P(MTM85-co-VFc15) film via electropolymerization of pyrrole in the presence of enzyme. The aim of the study is amperometric determination of various phenolics and investigation of the effect of interfacial interactions between enzyme and matrices on biosensor response.