Promising anticancer activity of a lichen, Parmelia sulcata Taylor, against breast cancer cell lines and genotoxic effect on human lymphocytes


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Arı F., Ulukaya E., Oran S., Çelikler Kasımoğulları S., Öztürk Ş., Ozel M. Z.

CYTOTECHNOLOGY, vol.67, no.3, pp.531-543, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 3
  • Publication Date: 2015
  • Doi Number: 10.1007/s10616-014-9713-4
  • Journal Name: CYTOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.531-543
  • Keywords: Parmelia sulcata, Cell death, Apoptosis, Breast cancer, Treatment, DNA damage, IN-VITRO, CYTOTOXIC ACTIVITIES, ANTIOXIDANT, METABOLITES, EXTRACTS, ASSAY, MURINE
  • Bursa Uludag University Affiliated: Yes

Abstract

Plants are still to be explored for new anticancer compounds because overall success in cancer treatment is still not satisfactory. As a new possible source for such compounds, the lichens are recently taking a great attention. We, therefore, explored both the genotoxic and anti-growth properties of lichen species Parmelia sulcata Taylor. The chemical composition of P. sulcata was analyzed with comprehensive gas chromatography-time of flight mass spectrometry. Anti-growth effect was tested in human breast cancer cell lines (MCF-7 and MDA-MB-231) by the MTT and ATP viability assays, while the genotoxic activity was studied by assays for micronucleus, chromosomal aberration and DNA fragmentation in human lymphocytes culture. Cell death modes (apoptosis/necrosis) were morphologically assessed. P. sulcata inhibited the growth in a dose-dependent manner up to a dose of 100 mu g/ml and induced caspase-independent apoptosis. It also showed genotoxic activity at doses (>125 mu g/ml) higher than that required for apoptosis. These results suggest that P. sulcata may induce caspase-independent apoptotic cell death at lower doses, while it may be genotoxic at relatively higher doses.