MOLECULAR SUBTYPING OF VANCOMYCIN RESISTANT ENTEROCOCCUS: A COMPARISON OF TWO MOLECULAR METHODS

KARAKEÇİLİ F., Cilo B. D., Karagoz A., Jefferies M., Cikman A., Akalin H., ...More

ACTA MEDICA MEDITERRANEA, vol.32, no.6, pp.1797-1803, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 6
  • Publication Date: 2016
  • Doi Number: 10.19193/0393-6384_2016_6_166
  • Journal Name: ACTA MEDICA MEDITERRANEA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1797-1803
  • Keywords: AP-PCR, molecular methods, PFGE, VRE, FIELD GEL-ELECTROPHORESIS, FAECIUM, PCR, INFECTIONS, EPIDEMIOLOGY, OUTBREAK, MLST
  • Bursa Uludag University Affiliated: Yes

Abstract

Introduction: Arbitrarily Primed-Polymerase Chain Reaction (AP-PCR) and Pulsed-Field Gel Electrophoresis (PFGE) are widely used genotyping methods for investigating outbreaks of infections. The aim of this research is to compare AP-PCR with PFGE, which is known as the gold standard method, and to determine if AP-PCR is suitable for use in outbreaks.