Both stress and burnout among employees are serious workplace problems. Work stress and burnout also affect employees' feelings towards work and their well-being. Employees' positive feelings towards work determine their attitude towards general life. For this reason, developing positive feelings towards work can also lead to a decrease in the stress and burnout experienced by the employee In this study, the relationship between burnout and job-related affective well-being among public sector employees was examined. In addition, this study considers whether job stress has a mediator effect between these two variables. 172 people participated in the study using the questionnaire method. Pearson Correlation Analysis was performed for the relationship between variables. in order to determine the mediator effect analyzes were performed in Structural Equation Modeling. According to the results of the analysis, there is a significant and negative relationship between job related affective well-being and burnout; significant and clear relationship between work stress and burnout In addition, the stress variable was found to play a partial mediator role in explaining the relationship between job-related affective well-being and burnout in other words, it is necessary to understand stress in order to explain the relationship between job well-being and perceptions of employees and burnout.