In Vitro Effects of Some Antibiotics on Purified β-Glucosidases from Rat Liver and Kidney Tissues

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Kara H., Türkmen Alemdar N.

ANTIBIOTICS, cilt.14, sa.6, ss.563-579, 2025 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 6
  • Basım Tarihi: 2025
  • Doi Numarası: 10.3390/antibiotics14060563
  • Dergi Adı: ANTIBIOTICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, EMBASE, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.563-579
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Abstract: Background: Antibiotics are antimicrobial drugs used to treat and prevent

infections. Unconscious use of antibiotics leads to many negative results. This study

aimed to emphasize the negative aspects of antibiotics by determining their effects on

purified enzymes. Methods: Beta glucosidase enzymes (BGLs) were purified from rat

liver and kidney tissues using the sepharose-4B-LTyrosine-1-Naphthylamine hydrophobic

interaction chromatography method. Liver BGL enzyme was purified 30.2-fold with a yield

of 43.4%, while kidney BGL was purified 5.1-fold with a yield of 12.2%. Purified enzymes

were visualized using SDS–PAGE. In vitro inhibition effects of ampicillin cefuroxime,

amoxicillin–clavulanate, cefazolin sodium, gentamicin, and ceftriaxone antibiotics were

determined on purified BGLs. Results: Ampicillin was found to inhibit rat liver and

kidney BGLs competitively and uncompetitively, with IC50 values of 69.56 and 25.30 mM,

respectively. Other antibiotics investigated did not significantly reduce liver BGL activity.

Cefuroxime inhibited rat kidney BGL uncompetitively with IC50 values of 76.88 mM, while

amoxicillin–clavulanate and cefazolin sodium inhibited it noncompetitively, with IC50

values of 41.32 and 98.81 mM, respectively. Gentamicin and ceftriaxone, whose effects were

investigated, did not reduce kidney BGL activity. Conclusions: Some of the commonly

used antibiotics reduce liver and kidney BGL activity, and this indicates that they may

potentially impair metabolic functions. These results emphasize that caution should be

exercised when using antibiotics.

Keywords: antibiotics; β-glucosidase; inhibition; rat; liver; kidney