Effects of Various Cryoprotective Agents on Post-Thaw Drone Semen Quality

Alcay S., Ustuner B., ÇAKMAK İ., Cakmak S., Nur Z.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.21, no.1, pp.31-35, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 1
  • Publication Date: 2015
  • Doi Number: 10.9775/kvfd.2014.11515
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.31-35
  • Keywords: Drone spermatozoa, Cryoprotectants, Cryopreservation, HONEY-BEE QUEENS, CRYOPRESERVATION, HYMENOPTERA, EXTENDER
  • Bursa Uludag University Affiliated: Yes


The aim of the present study was to evaluate the effect of different cryoprotectants on post-thaw semen motility and plasma membrane integrity of drone spermatozoa. Semen was obtained from mature drones (16 days or older) harvested from four colonies. Collected semen was diluted to a final concentration of 1/5 (semen/extender) in 0% cryoprotectant (control), 6% glycerol, 6% Ethylene Glycol, 6% 1,2 propanediol or 6% DMSO using a two-step dilution method. The equilibrated semen was frozen in 0.25-ml straws. The percentage of sperm motility and swollen tails (HOST) spermatozoa were evaluated following dilution with extender A (non-cryoprotectant), equilibration and post-thaw stages. In terms of post-thaw motility and plasma membrane integrity recovery we can rank the used cryoprotectant as DMSO, Ethylene Glycol, Glycerol and 1,2 Propanediol; respectively. In conclusion, post-thaw sperm motility and plasma membrane integrity of the present study was significantly better when sperm was frozen in DMSO with respect to control, glycerol, ethylene glycol, 1,2 propanediol (P<0.05).