DETERMINATION OF DIFFERENTIALLY EXPRESSED LATE RESPONSIVE GENES UPON TREATMENTS OF BTH, BABA AND TRICHODERMA HARZIANUM IN WHEAT

ERSOY F., Al-Asbahi A. A. S., Akkaya M. S.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.30, ss.7891-7897, 2021 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30
  • Basım Tarihi: 2021
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.7891-7897
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Chemically and biologically induced genes are involved in complex sets of defense responses against a broad spectrum of pathogens. Understanding the cross-link between specific and non-specific responses, systemic acquired resistance (SAR), upstream and downstream signaling molecules that regulate the transduction pathway is essential to solve response mechanisms. The wheat line Avocet-S was treated with benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester (BTH), beta-aminobutyric acid (BABA) and Trichoderma harzianum Raifi KRL-AG2, potentially used as bio-control fungal agent, for identification of candidate genes/gene fragments induced/repressed upon treatments. The differentially expressed wheat genes were detected by Differential Display RTPCR (DDRT-PCR), then cloned and sequenced. GenBank database was searched for significant hits of known genes using blastn algorithm. To verify the differences in the expressions of these gene fragments quantitative RT-PCR (qRT-PCR) was conducted. The confirmed differentially expressed wheat genes are Triticum aestivum Ubiquitin conjugating enzyme (TaUBC2), T. aestivum inducible phenylalanine ammonia-lyase (TaPAL1), T.aestivum autophagy-related protein 8 (TaATG8) and an unknown protein (TR1AE_CS42_4AL_TGACv1_288542_AA0951670.1). These findings are useful for identification of novel mechanisms modulating the defense-signaling network. Autophagy, ubiquitination and phenylpropanoid metabolism are important for immune responses and they can be activated by nonpathogenic chemicals and biological agents.