In this study, thermo-physiological comfort properties of woven fabrics produced with % 100 acrylic, cotton, viscose and PES weft as well as acrylic/PES, acrylic/cotton and acrylic/viscose weft mixing were investigated. For this purpose, 35 different fabrics were woven with acrylic warp and acrylic, cotton, PES, viscose, acrylic/cotton, acrylic/viscose and acrylic/PES weft yarns, with three different weft densities and plain, twill and satin weaves. Analysis of results showed that the effect of yarn material and weft density on thermal resistance was not observed. It was seen that weave type had an effect on thermal resistance, thermal absorbtivity and air permeability of the fabrics. Air permeability values of fabrics woven with plain weave were lower than those of satin and twill weaves. The air permeability decreased when weft density was increased. According to statistical evaluation, yarn type or yarn material had significant effect and weft density and weave had no significant effect on water vapour resistance.