Comparison of in vitro gas production, metabolizable energy, organic matter digestibility and microbial protein production of some legume hays

Karabulut A., Canbolat Ö., Kalkan H., Gurbuzol F., Sucu E., Filya İ.

ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, vol.20, no.4, pp.517-522, 2007 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 4
  • Publication Date: 2007
  • Doi Number: 10.5713/ajas.2007.517
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.517-522
  • Keywords: legume hay, gas production, digestibility, metabolizable energy, microbial protein, CHEMICAL-COMPOSITION, RUMEN, FERMENTATION, YIELD, DIET
  • Bursa Uludag University Affiliated: Yes


The aim of this study was to compare in vitro gas production kinetics, metabolizable energy (ME), organic matter digestibility (OMD) and microbial protein (MP) production of widely used legume hays in ruminant nutrition in Turkey. Gas production were determined at 0, 3 6, 12, 24, 48, 72 and 96 h and their kinetics were described using the equation p = a+b (1-e(-ct)). There were significant differences among legume hays in terms of chemical composition. The crude protein content of legume hays ranged from 11.7 to 18.6% of dry matter (DM); crude fat from 2.1 to 3.5% DM; neutral detergent fiber from 35.6 to 52.0% DM; acid detergent fiber from 32.0 to 35.5% DM and acid detergent lignin 1.7 to 11.0% DM. Total gas production after 96 h incubation ranged between 61.67 and 76.00 ml/0.200 g of substrate. At 24, 72 and 96 h incubation the total gas production for common vetch were significantly (p < 0.01) higher than those of the other legume hays. The ME, OMD and MP of legume hays ranged from 9.09 to 11.12 MJ/kg DM, 61.30 to 75.54% and 90.35 to 138.05 g/kg DM, respectively. The ME, OMD and MP of common vetch was significantly (p < 0.01) higher than those of the other hays due to low cell-wall contents and high crude protein. At the end of the experiment, differences in chemical composition of legume hays resulted in the differences in the in vitro gas production, gas production kinetics and the estimated parameters such as ME, OMD and MP. Common vetch can be recommended to hay producers and ruminant breeders, due to high ME, OMD and MP production.