Zn(ii), Cd(ii) and Hg(ii) saccharinate complexes with 2,6-bis(2-benzimidazolyl)pyridine as promising anticancer agents in breast and lung cancer cell linesviaROS-induced apoptosis


İÇSEL C., YILMAZ V. T. , Aydinlik S., AYGÜN M.

DALTON TRANSACTIONS, vol.49, no.23, pp.7842-7851, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 23
  • Publication Date: 2020
  • Doi Number: 10.1039/d0dt01535k
  • Journal Name: DALTON TRANSACTIONS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), Chimica, Communication Abstracts, Compendex, EMBASE, MEDLINE
  • Page Numbers: pp.7842-7851
  • Bursa Uludag University Affiliated: Yes

Abstract

New Zn(ii), Cd(ii) and Hg(ii) complexes of saccharinate (sac) and 2,6-bis(2-benzimidazolyl)pyridine (bzimpy), [Zn(bzimpy)(2)](sac)(2)center dot 2H(2)O (Zn), [Cd(sac)(2)(bzimpy)] (Cd) and [Hg(sac)(2)(bzimpy)] (Hg), were prepared and fully characterized by spectroscopic methods and X-ray crystallography.In vitroanticancer screening in A549 (lung), MCF-7 (breast) and HT29 (colon) cell lines showed thatZnwas highly cytotoxic against A549 and MCF-7 cells with IC(50)values of 1.74 +/- 0.06 and 3.15 +/- 0.10 mu M, respectively, andHgdemonstrated potent cytotoxic activity in MCF-7 cells (8.61 +/- 0.98 mu M), whileCdand bzimpy exhibited moderate growth inhibitory activities in all of the cell lines. In addition, they showed significantly lower toxicity towards normal human breast epithelial MCF10A cells. Moreover, the complexes exhibited significantly high nuclease activity towards plasmid DNA and their interactions with DNA were assessed by gel electrophoresis and DNA docking.ZnandHginduced G0/G1 cell arrest and apoptotic cell death detectedviatypical DNA condensation/fragmentation, annexin V staining and caspase 3/7 activity in A549 and MCF-7 cells. These complexes further caused depolarization of mitochondria and oxidative damage of genomic DNA following excessive production of reactive oxygen species (ROS).