The aim of this study was to determine aflatoxin B, (AFB(1)), T-2 toxin, fumonisin, deoxynivalenol (DON) and zearalenone (ZEN) levels in various feedstuffs (local and imported feed materials) and feed produced in a feed manufacturer. In addition, aflatoxin M-1 (AFM(1)) levels were determined in raw cow milks (n = 24) collected from 10 certain dairy cattle farms. The samples consisted of dairy cattle (n = 26), fattening (n = 4), and calf starter (n = 4) feed, as well as feedstuffs (n = 40). All of the samples were analysed by ELISA. In the analysed samples, the incidence of AFB(1), AFM(1) and T-2 toxins, fumonisin, DON and ZEN were 100%, 80%, 75% and 33%, respectively. The results of this study in feedstuffs and feed revealed that the mycotoxin levels were lower than the mycotoxin limits of the European Union (EU) and Turkey. In previous studies, high concentrations of mycotoxins, especially AFB(1) and AFM(1), were identified in feedstuffs, feeds and milk in Turkey. In contrast, the mycotoxin levels in the current study were lower than that from the previous studies. The mycotoxin concentrations in imported feed materials (except fumonisin) were lower than those in feed materials obtained from Turkey. The results of this study indicate that the mean mycotoxin levels in feedstuff and feeds do not cause toxic effects in animals, and humans for AFM(1).