Akademik Veri Yönetim Sistemi
6th European Congress of Immunology, İstanbul, Türkiye, 1 - 04 Eylül 2021, ss.185
Young infants respond relatively poorly to many infections and vaccines, but the basis of reduced immunity in infants is not completely defined. Mature neutrophils and granulocyticmyeloid
derived suppressor cells (G‐MDSC) can express similar cell surface markers. However, G‐MDSCs are functionally distinguished from neutrophils by its immunosuppressive
capacity on T cell responses. The literature regards G‐MDSCs as low density cells and indicate that they accumulate to upper 1.077g/mL Ficoll fraction. In this study, we aimed to
investigate low and high density of neutrophils that were accumulated both upper and lower phase of 1.077g/mL Ficoll phase Leukocytes were obtained from upper or lower 1.077g/
mL ficoll phase of children (age range from 5 days to 15 years).High and low density neutrophils were positively selected using with CD66b microbeads by magnetic activated cell
sorting.Different CD66b surface expression levels were detected (dim, mod and high) and purified by floresen activating cell sorting (FACS).These subpopulations surface molecules
expression (CD15,CD14,CD33,CD16,HLA‐DR,CD114,CD62‐L,Lox‐1,PD‐L1,PD‐L2,CD80,CD86) were studied with flow cytometry.Dichlorodihydrofluorescein diacetate(DCFDA) was used
to measure ROS production.Carboxyfluorescein succinimidyl ester(CFSE) for proliferation analysis of CD4+ or CD8+ T cells obtained from healthy donors in the presence of low or
high density of neutrophils from healthy children.Haematoxylin‐eosin staining of blood smears were used for confirm maturation stages of neutrophils. Peripheral blood low‐density
neutrophils in early childhood stimulate T‐cell responses as much as high‐density neutrophils
This study is being supported by The Scientific and Technological Research Council of Turkey (TUBITAK), Project no. 120S653