Cytotoxic Potential of Rare Plant<i> Salvia</i><i> candidissima</i> subsp.<i> candidissima</i> on Breast Cancer Cells

Erturk E., Onur O. E., Aydin I., Ozel M. Z., Firat M., Arı F.

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, cilt.66, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 66
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1590/1678-4324-2023220358
  • Dergi Adı: BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Anahtar Kelimeler: Salvia candidissima Vahl, subsp, candidissima, breast cancer, MCF-7, MDA-MB-231, cytotoxicity, BETA-CARYOPHYLLENE, ESSENTIAL OIL, CHEMICAL-COMPOSITION, GROWTH-INHIBITION, MYRICA-RUBRA, APOPTOSIS, ROOTS, ANTIOXIDANT, INDUCTION, SCLAREOL
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Breast cancer is the leading cause of cancer-related deaths in women throughout the world. Research on natural anti-cancer products from plants has gained traction. Salvia L. species and their derivatives are rare in Turkey and have suggested for their potential anti-cancer effects. The aim of this study is to assess the potential cytotoxic/apoptotic activities of methanol extract of Salvia candidissima Vahl. subsp. candidissima (SCE) on MCF-7 and MDA-MB-231 breast cancer cells. A GCxGC-TOF/MS system and a dual stage commercial thermal desorption injector were used to determine the chemical components of SCE. MTT and ATP viability tests were used to investigate the anti-growth activity. The apoptosis-inducing effect was assessed using a fluorescence staining method. Caspase-cleaved keratin 18 (ccK18, M30-antigen) levels measured by M30-CytoDeath ELISA Kit. The results showed that SCE suppressed the survival of the MCF-7 and MDA-MB-231 breast cancer cells in a dose-dependent manner, based on the findings of both MTT and ATP cell viability tests and pyknotic cell nuclei were observed via fluorescent staining in both cell lines after 48 h of treatment. The treatment group had greater levels of caspase-cleaved keratin 18 in the MCF-7 cells than the untreated group. These results showed that SCE triggers apoptosis, causes cell death in MCF-7 and MDA-MB-231 cell lines. SCE may become promising therapeutic strategy in the treatment of breast cancer with further in vitro and in vivo studies.