Cytotoxic Potential of Rare Plant Salvia candidissima subsp. candidissima on Breast Cancer Cells


Erturk E., Onur O. E., Aydin I., Ozel M. Z., Firat M., Arı F.

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, vol.66, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 66
  • Publication Date: 2023
  • Doi Number: 10.1590/1678-4324-2023220358
  • Journal Name: BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Keywords: Salvia candidissima Vahl, subsp, candidissima, breast cancer, MCF-7, MDA-MB-231, cytotoxicity, BETA-CARYOPHYLLENE, ESSENTIAL OIL, CHEMICAL-COMPOSITION, GROWTH-INHIBITION, MYRICA-RUBRA, APOPTOSIS, ROOTS, ANTIOXIDANT, INDUCTION, SCLAREOL
  • Bursa Uludag University Affiliated: Yes

Abstract

Breast cancer is the leading cause of cancer-related deaths in women throughout the world. Research on natural anti-cancer products from plants has gained traction. Salvia L. species and their derivatives are rare in Turkey and have suggested for their potential anti-cancer effects. The aim of this study is to assess the potential cytotoxic/apoptotic activities of methanol extract of Salvia candidissima Vahl. subsp. candidissima (SCE) on MCF-7 and MDA-MB-231 breast cancer cells. A GCxGC-TOF/MS system and a dual stage commercial thermal desorption injector were used to determine the chemical components of SCE. MTT and ATP viability tests were used to investigate the anti-growth activity. The apoptosis-inducing effect was assessed using a fluorescence staining method. Caspase-cleaved keratin 18 (ccK18, M30-antigen) levels measured by M30-CytoDeath ELISA Kit. The results showed that SCE suppressed the survival of the MCF-7 and MDA-MB-231 breast cancer cells in a dose-dependent manner, based on the findings of both MTT and ATP cell viability tests and pyknotic cell nuclei were observed via fluorescent staining in both cell lines after 48 h of treatment. The treatment group had greater levels of caspase-cleaved keratin 18 in the MCF-7 cells than the untreated group. These results showed that SCE triggers apoptosis, causes cell death in MCF-7 and MDA-MB-231 cell lines. SCE may become promising therapeutic strategy in the treatment of breast cancer with further in vitro and in vivo studies.